Coding
Part:BBa_K1781004:Design
Designed by: Marvin Prein Group: iGEM15_TU_Dresden (2015-09-09)
T18 - one of two fragments of the T25/T18 BACTH system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 19
Illegal AgeI site found at 574 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We removed the multiple cloning site within the T18 so that it did not interfere with the prefix and suffix
Source
The sequence was retrieved from the Euromedex BACTH System Kit